Medium 17: R2A Agar (Reasoner’s 2A agar)

Composition:
– Casein peptone – 0.5 g
– Meat peptone – 0.5 g
– Yeast extract – 0.5 g
– Glucose – 0.5 g
– Soluble starch – 0.5 g
– Na₂H₂P₂O₇ – 0.3 g
– MgSO₄·7H₂O – 0.05 g
– KH₂PO₄ – 0.3 g
– Agar – 15.0 g
– Distilled water – 1 L

Sterilize at 0.5–0.7 atm for 45 minutes, pH 7.2 ± 0.2.