Medium 08: Tyrosine Agar (ISP 7)

Composition:
– Glycerol – 15.0 g
– L-Aspartic acid – 1.0 g
– NaCl – 0.5 g
– L-Tyrosine – 0.5 g
– K₂HPO₄ (anhydrous) – 0.5 g
– MgSO₄·7H₂O – 0.5 g
– Agar – 18.0 g
– Distilled water – 1 L
– Microelement solution – 1 ml

Adjust pH to 7.5. Sterilize at 100°C for 10–20 minutes.