M C C A P

Medium 05: Inorganic Salt Starch Agar (ISP-4)

Solution I:
– Soluble starch – 10.0 g, mix with a small volume of distilled water to form a paste.
– Add distilled water to bring the total volume to 500 ml.

Solution II:
– CaCO₃ – 2.0 g
– K₂HPO₄ (anhydrous) – 1.0 g
– MgSO₄ × 7H₂O – 1.0 g
– NaCl – 1.0 g
– (NH₄)₂SO₄ – 2.0 g
– Distilled water – 500.0 ml
– Salt solution (see Medium 1) – 1.0 ml

Add Solution II to Solution I, then add agar (20.0 g).
Sterilize at 100°C for 10–20 minutes.
Adjust pH to 7.0–7.4.