Medium 06: Glycerol-Asparagine Agar (ISP-5)

Composition:

L-Asparagine (anhydrous) – 1.0 g
Glycerol – 10.0 g
K₂HPO₄ (anhydrous) – 1.0 g
Distilled water – 1 L
Salt solution (see Medium 1) – 1.0 ml
Agar – 20.0 g

Dissolve the components in water in the specified order.
Sterilize at 100°C for 10–20 minutes.
pH 7.0–7.4.