Medium 07: Peptone Yeast Extract Iron Agar (ISP 6)

Composition:
– Peptone – 10.0 g
– Yeast extract – 1.0 g
– FeSO₄·7H₂O – 0.5 g
– Agar – 18.0 g
– Distilled water – 1 L

Sterilize at 100°C for 10–20 minutes, pH 7.0–7.2.